Composite

Part:BBa_K3425070:Design

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-26)


Modular outer membrane display construct for nanobody specific to HSP biomarker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1036
    Illegal AgeI site found at 625
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is composed of three BioBricks tailored for assembly by iGEM Type IIS standard:
BBa_K3425042
BBa_K3425043
BBa_K3425044

It is a modular system for the exposure of nanobodies on the outer surface of E. coli. In this case, the nanobody BBa_K3425043 is specific for HSP protein, biomarker of Mycobacterium tuberculosis. However, parts are designed so that the nanobody can be easily swapped. Codon optimised for E. coli.

Source

Registry of Standard Biological Parts
BBa_K3425042
BBa_K3425043
BBa_K3425044

References